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Year : 2015  |  Volume : 4  |  Issue : 3  |  Page : 244-247

Same day sputum smear microscopy with an economical destaining step for the diagnosis of pulmonary tuberculosis

1 Department of Microbiology, Sathyabama University, Chennai, India
2 Centre for Laboratory Animal Technology and Research (CLATR), Sathyabama University, Chennai, India
3 Department of Pathology, GSL Medical College, Rajahmundry, Sathyabama University, Chennai, India

Correspondence Address:
T Jaya Chandra
Lecturer, Department of Microbiology, Coordinator, Central Research Laboratory, GSL Medical College, Rajahmundry
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Source of Support: None, Conflict of Interest: None

DOI: 10.15380/2277-5706.JCSR.15.019

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Back ground: Acid fast bacilli (AFB) positivity using modified Ziehl Neelsen (MZN) staining and jar method was studied in sputum samples obtained by spot morning (SM) and same day (SS2) approaches. Methods: Three specimens (spot, second spot and morning) were collected in 252 patients, duplicate smears were prepared and stained by MZN staining and jar method. Results: Smear positivity was 34 (13.5%) and 33 (13%) for MZN staining and jar method respectively (p>0.99) for the SM approach; and 34 (13.5%) and 32 (12.7%) for MZN staining and jar method (p>0.99) respectively for SS2 approach. Conclusion: Jar method in view of lesser cost and similar diagnostic yielf can be useful for training purposes.

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